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Merck
모든 사진(1)

문서

SRP3171

Sigma-Aldrich

TGF-beta 3-E. coli human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

동의어(들):

Transforming Growth Factor-beta3

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About This Item

UNSPSC 코드:
12352202
NACRES:
NA.32

생물학적 소스

human

재조합

expressed in E. coli

분석

≥98% (HPLC)
≥98% (SDS-PAGE)

형태

lyophilized

효능

≤0.05 ng/mL ED50

분자량

25.0 kDa

포장

pkg of 10 μg

기술

cell culture | mammalian: suitable

불순물

<0.1 EU/μg endotoxin, tested

색상

white

UniProt 수납 번호

배송 상태

wet ice

저장 온도

−20°C

유전자 정보

human ... TGFB3(7043)

일반 설명

The gene TGF-β3 (transforming growth factor β 3) is mapped to human chromosome 14q24.3. The three mammalian isoforms of TGF-β, TGF-β1, β2, β3 elicit similar biological responses. TGF-β proteins are made as precursor molecules which are cut to generate a mature carboxy-terminal region of 110-140 amino acids. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. Recombinant TGF-β3 is a 25.0kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

애플리케이션

TGF-β3 (transforming growth factor β 3) human has been used in the media for the chondrogenic differentiation of the stromal cells. It has been used in the culture medium to allow attachment of primary human dermal fibroblasts to the scaffolds.

생화학적/생리학적 작용

The three mammalian isoforms of TGF-β (transforming growth factor β), TGF-β1, β2, β3, are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. TGF-β3 is the predominant TGF-β in the testis and controls the blood-testis barrier activity. Polymorphisms in this gene might be associated with male infertility. Heterozygous mutation in the gene at position Arg300 might be associated with increased TGF-β signaling, leading to a disorder with phenotypic overlap with Marfan syndrome and Loeys-Dietz syndrome. TGF-β3 is involved in the mesenchymal stem cells (MSCs)-mediated inhibition of fibroblast proliferation and decreases skin fibrosis.

서열

ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

물리적 형태

Lyophilized from 0.1% TFA

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 °C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 °C to -80 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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