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Merck
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TP0010

Millipore

FLAG® HA Tandem Affinity Purification Kit

동의어(들):

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

일반 설명

TAP technology is a recent development in protein purification. This technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification of protein complexes by adding a tandem affinity tag to a known "bait" protein to pull down endogenous proteins that interact with the targeted protein of interest.

애플리케이션

The FLAG® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology.

Sutitable for mass spectrometry analysis and western blotting.

Learn more product details in our FLAG® application portal.

법적 정보

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS
  • F2426EZview™ Red ANTI-FLAG® M2 Affinity Gel, clone M2SDS
  • A2095Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspensionSDS
  • R0278RIPA BufferSDS
  • F47993X FLAG® Peptide, lyophilized powderSDS
  • U4883Urea, 8 M (after reconstitution with 16 mL high purity water)SDS

픽토그램

Exclamation markEnvironment
GHS07,GHS09

신호어

Warning

유해 및 위험 성명서

H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Junsong Zhang et al.
Journal of virology, 90(8), 3966-3980 (2016-02-05)
The viral ribonucleoprotein (vRNP) complex of influenza A viruses (IAVs) contains an RNA-dependent RNA polymerase complex (RdRp) and nucleoprotein (NP) and is the functional unit for viral RNA transcription and replication. The vRNP complex is an important determinant of virus
Wei Li et al.
Cell, 140(4), 477-490 (2010-02-25)
Current models imply that the FERM domain protein Merlin, encoded by the tumor suppressor NF2, inhibits mitogenic signaling at or near the plasma membrane. Here, we show that the closed, growth-inhibitory form of Merlin accumulates in the nucleus, binds to
Karthik Ramachandran et al.
iScience, 25(1), 103722-103722 (2022-01-11)
SARS-CoV-2 is a newly identified coronavirus that causes the respiratory disease called coronavirus disease 2019 (COVID-19). With an urgent need for therapeutics, we lack a full understanding of the molecular basis of SARS-CoV-2-induced cellular damage and disease progression. Here, we
Kosei Sato et al.
Nature communications, 10(1), 166-166 (2019-01-13)
In Drosophila, some neurons develop sex-specific neurites that contribute to dimorphic circuits for sex-specific behavior. As opposed to the idea that the sexual dichotomy in transcriptional profiles produced by a sex-specific factor underlies such sex differences, we discovered that the

문서

FLAG® HA System

The FLAG® HA tandem epitope system consists of small epitopes tags that are not eukaryotic derived. These features minimize interference with protein functions and provide superior specificity.

관련 콘텐츠

단백질 정제

이온교환, 크기 배제 및 단백질 친화성 크로마토그래피를 포함한 방법을 사용하는 재조합 단백질 정제를 위한 단백질 정제 기법, 시약 및 프로토콜.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein & Nucleic Acid Interactions

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

단백질 및 핵산 상호작용

단백질 및 핵산 상호작용 시약과 단백질-RNA, 단백질-DNA, 단백질-단백질 상호작용 및 관련 애플리케이션 투자용 관련 자료

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