ZRB1153

Anti-53BP1 Antibody, clone 1H16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): TP53-binding protein 1, p53-binding protein 1, p53BP1
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 클론: recombinant monoclonal
• 제품 라인: ZooMAb^® learn more
• 형태: lyophilized
• 분자량: calculated mol wt 213.57 kDa; observed mol wt ~270 kDa
• 종 반응성: mouse, human
• 포장: antibody small pack of 25 μL
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 동형: IgG
• UniProt 수납 번호: Q12888
• 환경친화적 대안 카테고리: , Aligned
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

특이성

Clone 1H16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects p53-binding protein 1. It targets an epitope within the N-terminal half.

면역원

His-tagged recombinant fragment corresponding to 250 amino acids from the N-terminal half of human p53-binding protein 1 (53BP1).

애플리케이션

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected 53BP1 in HEK293 cell nuclear extract.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected 53BP1 in HeLa cell Nuclear Extract.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected 53BP1 in human kidney tissue sections.Affinity Binding Assay: A representative lot of this antibody bound recombinant 53BP1 protein fragment with a KD of 1.2 x 10-8 in an affinity binding assay. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected 53BP1 in HeLa, A431, HepG2, NIH 3T3 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-53BP1, clone 1H16 ZooMAb, Cat. No. ZRB1153, is a recombinant Rabbit monoclonal antibody that specifically targets 53BP1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

표적 설명

TP53-binding protein 1 (UniProt: Q12888; also known as 53BP1, p53-binding protein 1, p53BP1) is encoded by the TP53BP1 gene (Gene ID: 7158) in human. 53BP1 is a homooligomeric double-strand break (DSB) repair protein that is involved in response to DNA damage and promotes non-homologous end joining (NHEJ)-mediated repair. It counteracts the function of the homologous recombination (HR) repair protein BRCA1. In response to DSB it undergoes phosphorylation by ATM, which promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites where it binds histone H2A monoubiquinated at lysine 15 (H2AK15Ub) and histone H4 dimethylated at lysine 20 (H4K20me2). Its Tudor-like region (aa 1484-1603) mediates its binding to H4K20m3e2. In the absence of DNA damage, it is phosphorylated at basal level, but following DSB and phosphorylation by ATM it interacts with PAX-interacting protein 1 (PAXIP1) and non-homologous end joining of dysfunctional telomeres. Three isoforms of 53BP1 have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Panier, S., and Boulton, S. (2014). Nat Rev Mol Cell Biol 15(1); 7-18).

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

재구성

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable