콘텐츠로 건너뛰기
Merck
모든 사진(12)

Key Documents

ZRB1298

Sigma-Aldrich

Anti-CD28 Antibody, clone 2N15 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

재조합

expressed in HEK 293 cells

결합

unconjugated

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

2N15, recombinant monoclonal

설명

recombinant, expressed in HEK 293 cells

제품 라인

ZooMAb® learn more

형태

lyophilized

분자량

calculated mol wt 25.07 kDa
observed mol wt ~40 kDa

정제법

using Protein A

종 반응성

human

종 반응성(상동성에 의해 예측)

bovine, monkey, rat

포장

antibody small pack of 25 μL

환경친화적 대안 제품 특성

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

향상된 검증

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

기술

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

동형

IgG

에피토프 서열

C-terminal half

단백질 ID 수납 번호

UniProt 수납 번호

환경친화적 대안 카테고리

배송 상태

ambient

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

특이성

Clone 2N15 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects CD28. It targets an epitope within 15 amino acids from the extracellular domain.

면역원

KLH-conjugated linear peptide corresponding to 15 amino acids from the extracellular domain of human CD28.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in Human tonsil tissue lsyate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CD28 in Human tonsil tissue lysate.

Tested applications

Immunocytochemistry Analysis: A 1:40 dilution from a representative lot detected CD28 in Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD28 in human tonsil tissue sections.

Flow Cytometry Analysis: 1 μg from a representative lot detected CD28 in one million human peripheral blood mononuclear cells (PBMC).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CD28, clone 2N15 ZooMAb®, Cat. No. ZRB1298, is a recombinant Rabbit monoclonal antibody that detects CD28 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

표적 설명

T-cell-specific surface glycoprotein CD28 (UniProt: P10747; also known as TP44, CD28) is encoded by the CD28 gene (Gene ID: 940) in human. CD28 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 19-152), a transmembrane domain (aa 152-179), and a cytoplasmic domain (aa 180-220). It is mainly expressed on T lineage cells, including thymocytes, but may also be found on plasmablasts. It is reported to be constitutively expressed on both resting and activated T cells, on almost all CD4+ and on at least half of CD8+ T cells in humans. It is involved in T cell activation, proliferation, survival, and cytokine production. It reduces the threshold of TCR triggering required for T cell activation. CD28 signaling transduces a positive signal that promotes proliferation of TCR-stimulated T cells. It is shown to cause the initial activation of naïve CD4 T cells by increasing the sensitivity of T cells to antigen receptor engagement. It also enhances the production of IL-4 and IL-10 in T-cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. Upon activation of mature T cells, either by mitogens or by cross-linking of the TCR, the expression of CD28 is shown to increase. Ligation of CD3 and CD28 (CD3/28 co-stimulation) is shown to promote increase in glucose metabolism, increased cytokine/chemokine expression, elevate IL-2 production, and induce resistance to apoptosis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Riley, JL., and June, CH. (2005). Blood. 105(1); 13-21).

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

재구성

1.2 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

암의 특징에 대해 알아보고, 암 특징별로 편리하게 정리된 신뢰할 수 있는 ZooMAb® 재조합형 항체를 확인해보세요.

Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.