추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
1H17, monoclonal
recombinant monoclonal
제품 라인
ZooMAb® learn more
형태
lyophilized
분자량
calculated mol wt ~82.56 kDa
observed mol wt ~105 kDa
종 반응성
rat, mouse, human
종 반응성(상동성에 의해 예측)
monkey, bovine
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
특이성
Clone 1H17 is a ZooMAb® rabbit recombinant monoclonal antibody that detects DNA-binding protein SATB2. It targets an epitope within 21 amino acids from the C-terminal half.
면역원
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal half of human DNA-binding protein SATB2.
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in K562 cell nuclear extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SATB2 in K562 cells nuclear extract.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected SATB2 in Mouse and Rat brain nuclear extract.
Immunocytochemistry Analysis (ICC): A 1:100 dilution from a representative lot detected SATB2 in HeLa, A431, and HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SATB2 in human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound SATB2 peptides with a KD of 1.0 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in K562 cell nuclear extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SATB2 in K562 cells nuclear extract.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected SATB2 in Mouse and Rat brain nuclear extract.
Immunocytochemistry Analysis (ICC): A 1:100 dilution from a representative lot detected SATB2 in HeLa, A431, and HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SATB2 in human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound SATB2 peptides with a KD of 1.0 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SATB2, clone 1H17 ZooMAb®, Cat. No. ZRB1342, is a recombinant Rabbit monoclonal antibody that specifically targets SATB2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
표적 설명
DNA-binding protein SATB2 (UniProt: Q9UPW6; also known as Special AT-rich sequence-binding protein 2, SATB2) is encoded by the SATB2 (also known as KIAA1034) gene (Gene ID: 23314) in human. SATB2 serves as a transcription factor controlling nuclear gene expression by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. It recognizes the sugar-phosphate structure of double-stranded DNA. It acts as a docking site for several chromatin remodeling enzymes and recruits corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. SATB2 contains two 2 CUT DNA-binding domains (aa 350-437 and 473-560) and a classic homeobox DNA binding domain (aa 615-674). It is highly expressed in the adult brain and its moderate expression has been reported in fetal brain. Postnatally, it is required for proper dendritic and soma adhesion of callosal neurons in neocortex and its deletion is reported to cause impairment in long-term memory. Mutations in SATB2 gene have been linked to cleft palate condition. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhang, L., et al. (2020). Cell Death Differ. 27(5); 1604-1617).
물리적 형태
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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