기술
HPLC: suitable
검사 파라미터
column: Ascentis Express Phenyl-Hexyl, 15 cm x 2.1 mm, 2.7 μm particles (53338-U)
mobile phase: [A] 70:30, water with 0.1% TFA:acetonitrile with 0.09% TFA; [B] 55:45, water with 0.1% TFA:acetonitrile with 0.09% TFA
gradient: 0 to 100% B in 7.5 min
flow rate: 0.3 mL/min
pressure: 3960 psi (273 bar)
column temp.: 30 °C
detector: UV, 215 nm
injection: 0.5 μL
sample: 200 μg/mL in 0.1% formic acid
적합성
application for HPLC
응용 분야
life science and biopharma
pharmaceutical (small molecule)
분석 메모
These three insulins have more than 76% identical positions in a primary structure alignment. The human and porcine proteins are most similar with 85% identity. The Phenyl-Hexyl phase provides an alternate retention and selectivity to a C18 phase, particularly the C18 phase on a larger pore silica, Ascentis Express ES-C18
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
분석물
설명
Insulin from bovine pancreas, ≥25 USP units/mg (HPLC), powder
Insulin human, powder, recombinant, expressed in E. coli, suitable for cell culture
Insulin from porcine pancreas, powder, ≥27 USP units/mg
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