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  • Global profiling of co- and post-translationally N-myristoylated proteomes in human cells.

Global profiling of co- and post-translationally N-myristoylated proteomes in human cells.

Nature communications (2014-09-27)
Emmanuelle Thinon, Remigiusz A Serwa, Malgorzata Broncel, James A Brannigan, Ute Brassat, Megan H Wright, William P Heal, Anthony J Wilkinson, David J Mann, Edward W Tate
초록

Protein N-myristoylation is a ubiquitous co- and post-translational modification that has been implicated in the development and progression of a range of human diseases. Here, we report the global N-myristoylated proteome in human cells determined using quantitative chemical proteomics combined with potent and specific human N-myristoyltransferase (NMT) inhibition. Global quantification of N-myristoylation during normal growth or apoptosis allowed the identification of >100 N-myristoylated proteins, >95% of which are identified for the first time at endogenous levels. Furthermore, quantitative dose response for inhibition of N-myristoylation is determined for >70 substrates simultaneously across the proteome. Small-molecule inhibition through a conserved substrate-binding pocket is also demonstrated by solving the crystal structures of inhibitor-bound NMT1 and NMT2. The presented data substantially expand the known repertoire of co- and post-translational N-myristoylation in addition to validating tools for the pharmacological inhibition of NMT in living cells.