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HPLC Analysis of Phenylalanine Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive)

HPLC Analysis of Phenylalanine Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive) application for HPLC

Materials

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Astec® CHIROBIOTIC® T Chiral HPLC Guard Column

Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

분석 컬럼

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Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

표준

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L-Phenylalanine

from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%

L-Phenylalanine

reagent grade, ≥98%

D-Phenylalanine

≥98% (HPLC)

L-Phenylalanine

BioUltra, ≥99.0% (NT)

L-Phenylalanine

99%, FCC

L-Phenylalanine

analytical standard, for Nitrogen Determination According to Kjeldahl Method

L-Phenylalanine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 205 nm

injection

10 μL

sample

300 μg /mL in 30:70 water:methanol

설명

법적 정보

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC