HPLC Analysis of Triazole Antifungal Agent Enantiomers on Astec^® CYCLOBOND I 2000 HP-RSP

column

Astec CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)

mobile phase

[A] 0.1% formic acid; [B] acetonitrile; (70:30, A:B)

flow rate

1 mL/min

detector

UV, 230 nm

injection

1 μL

sample

0.1 mg/mL in methanol
peaks 1&2: enilconazole
peaks 2&3: eberconazole
peaks 4&5: miconazole
peaks 6&7: ketoconazole