EnPresso, a proprietary growth system from BioSilta Ltd, applies slow enzymatic glucose release to control the growth and protein synthesis rate in bacteria and yeasts. This high-cell-density growth system can be used in any shaken cultivation device at various scales. In this webinar, Dr. Kaisa Ukkonen and Dr. Antti Vasala will review the basic requirements for successful high-cell-density cultivations and discuss common reasons for failures in E. coli-based protein production, the focus being on the cultivation conditions. In particular, prerequisites for successful scale-up will be presented. The webinar also features examples on the use of EnPresso demonstrating the time and cost savings enabled by this powerful growth system, and presents an interesting alternative protein expression protocol: IPTG-based autoinduction.
Dr. Kaisa Ukkonen
Head of Protein Expression Technologies
Dr. Antti Vasala
R & D Director
Session 1:presented May 31, 2021
To continue reading please sign in or create an account.Don't Have An Account?