Duolink^® PLA Technology: How to detect and quantify protein webinar

WEBINAR

What Does it Cover?

Duolink^® proximity ligation assay (PLA) technology allows you to visualize protein interactions with cellular localization and quantities by amplifying signals corresponding to single and post-translational protein events. With 1000x sensitivity and high specificity, this protein detection technology allows to visualize protein functions, all within a native cell. The PLA method provides:

• Visual protein interactions
  − Both stable and transient
• Endogenous protein detection
  − No overexpression or genetic manipulation
• High specificity
  − Use of two antibodies/probes eliminates false positives
• Single molecule sensitivity
  − Rolling circle amplification makes proteins visible
• No special equipment needed
  − Standard immunofluorescence methods

This webinar will review how to work with PLA technology and provide an overview of their potential along with example applications.

What Will You Learn?

• How to work with Duolink^® PLA technology
• Overview of most relevant applications using Duolink^®
• Product offering to run an assay with PLA using Immunofluorescence or Flow Cytometry
• Benefits of working with highly specific and powerful technology to study protein biology

Who Should Attend?

Researchers in the T-Cell, adaptive immunotherapy, and immuno-oncology fields