The dysregulation of lipid metabolism is known to be associated with malignancy and metastatic progression in colorectal cancer (CRC). However, a detailed survey of the global ‘lipidomic hallmarks’ of CRC, and evaluation of whether variations in lipidome profiles between individual patient tumour samples constitute a distinct, or complementary, taxonomy relative to currently used genomic, transcriptomic or proteomic classifications, is currently lacking. Furthermore, conventional analytical strategies for lipidome analysis typically fail to provide sufficient information for precise structural characterization, and quantification, of the individual molecular lipid species that may be present within a complex sample of interest. To address these needs, I will introduce some of the key concepts of lipidomics, and then describe results from our recent efforts aimed at the development of an integrated quantitative workflow for ‘shotgun’ lipidome analysis, involving the use of stable-isotope labelled lipid standards, functional group selective derivatization and ultra-high resolution mass spectrometry and UV-photodissociation tandem mass spectrometry techniques, and the application of this workflow for the characterization of lipidome profile alterations in a series of clinical CRC tumor and normal tissue samples, and CRC cell lines.
Gavin E. Reid, PhD
University of Melbourne
Professor of Bioanalytical Chemistry
Dr. Gavin E. Reid is the Professor of Bioanalytical Chemistry in the School of Chemistry and the Department of Biochemistry and Molecular Biology, and member of the multidisciplinary Bio21 Molecular Science and Biotechnology Institute at The University of Melbourne, Australia. Over the past 33 years, he has held a variety of technical research positions and academic appointments in Australia and the USA. Driven by a belief that innovations in technology are enabling for biomedical discovery, research in the Reid laboratory is primarily directed toward the development of mass spectrometry based analytical chemistry strategies for quantitative proteome and lipidome analysis, and their application toward identifying the functional role of proteins and lipids in the regulation of cellular function, and in the onset and progression of disease, including cancer. To date, this inter-disciplinary research has resulted in 163 peer-reviewed publications, >160 conference and invited seminar presentations, and 4 patents. Gavin is currently President of the Australian and New Zealand Society for Mass Spectrometry, an Associate Editor for the Journal of the American Society for Mass Spectrometry, and is on the Editorial Advisory Boards for the Journal of Lipid Research, and the European Journal of Mass Spectrometry. He served from 2012-2014 on the Board of Directors of the American Society for Mass Spectrometry, and was Treasurer of the Australasian Proteomics Society from 2015-2017.
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