HomeWebinarsProcess Development and Intensification for Ultrafiltration/Diafiltration of Viral Vectors

Process Development and Intensification for Ultrafiltration/Diafiltration of Viral Vectors


Fast and efficient processes with reliable scale-up capabilities are essential for viral vector manufacturing. The primary tangential flow filtration (TFF) — step-through ultrafiltration and diafiltration (UF/DF) — can intensify the purification process prior to chromatography steps.

We will explore two operating strategies, transmembrane pressure (TMP) and permeate control, while considering their impact on process time, viral yield, and impurity removal. We will also compare the performance of commercially available single-use TFF modules for processing viral vectors. These insights offer a foundation for process development and optimization of processing viral vectors.

In this webinar, you will learn about:

  • The methods to develop, optimize and scale up a TFF process for viral vector manufacturing with different operating strategies
  • Intensifying the downstream purification process for viral vectors
  • Process performance characteristics of different single-use technologies available for viral vector manufacturing


Anand Alembath

Anand Alembath


Development Engineer

Anand Alembath is a development engineer and the applications technical lead for tangential flow filtration (TFF) product development projects with a focus on downstream purification of viral vectors. He holds an M.S. and Ph.D. in chemical engineering from Missouri University of Science and Technology, Rolla.

Rosario Cervellere

Rosario Cervellere


Applications Engineer

Rosario Cervellere is an applications engineer in filtration R&D. Since joining in June 2021, he has supported technology and product development projects including Pelicon® Capsule with 100 and 300 kDa Ultracel® membrane and Pellicon® XL 50 cassettes. His recent focus applies modeling and simulation to increase the efficiency of product and technology development. Rosario holds a Ph.D. in mechanical engineering from the University of Arkansas with a focus on computational materials science and global change.

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