What’s Really in the Box? Characterizing AAV Encapsidated Nucleic Acid Sequences Using NGS

WEBINAR

Due to the nature of AAV particle production, non-targeted sequences may be packaged along with the gene of interest. Even in an optimized system, these non-target nucleic acids can comprise between 1-2% or greater of the entire nucleic acid payload. Characterizing the non-targeted sequences that may be packaged in the vector is important for establishing the safety profile of the final product.

NGS can provide a clearer picture of all encapsidated sequences within the AAV particles. In this webinar, we discuss the characterization of several representative AAV molecules using NGS, including the sequencing process, bioinformatic analysis, and representative results.

In this webinar, we will explore:

• How NGS can provide broad characterization of targeted and non-targeted sequences packaged in an AAV particle
• The process for evaluating encapsidated residual sequences in an AAV sample
• Concepts for the determination of nucleic acid content of full, partial, and empty capsids