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S9430
SYBR® Green I nucleic acid gel stain
10,000 × in DMSO
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usage
mL sufficient for 100 mini-gels
Quality Level
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
concentration
10,000 × in DMSO
greener alternative category
storage temp.
−20°C
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This Item | S9305 | SCT124 | SCT125 |
|---|---|---|---|
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level - |
| usage mL sufficient for 100 mini-gels | usage mL sufficient for 100 mini-gels | usage - | usage - |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics - | greener alternative product characteristics - |
| greener alternative category | greener alternative category | greener alternative category - | greener alternative category - |
General description
Application
- the quantification of dsDNA
- to stain DNA in polymerase chain reaction (PCR)
- for comet assay technique
- to assess spermatozoon membrane integrity
- for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
- as a fluorescent dye in flow cytometry
- real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA
Features and Benefits
- An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
- It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
- It is less mutagenic than ethidium bromide in Ames tests
- It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
- Useful for many applications with a limited amount of DNA
- The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
- Removal of this stain in-gel digestion and ligation techniques is not needed
- SYBR Green I is a greener alternative product to ethidium bromide for staining
Storage and Stability
Legal Information
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
201.2 °F - closed cup
Flash Point(C)
94 °C - closed cup
Personal Protective Equipment
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