MilliporeSigma
All Photos(2)

Documents

05-1066

Sigma-Aldrich

Anti-Insulin Antibody, clone E11D7

clone E11D7, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-IDDM, Anti-IDDM1, Anti-IDDM2, Anti-ILPR, Anti-IRDN, Anti-MODY10, Anti-PNDM4
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

E11D7, monoclonal

species reactivity

human

species reactivity (predicted by homology)

rat, mouse

packaging

antibody small pack of 25 μg

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... INS(3630)
mouse ... Ins1(16333)
rat ... Ins1(24505)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
05-1108I8409WH0003418M1
antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

E11D7, monoclonal

clone

MAB1, monoclonal

clone

polyclonal

clone

5F11, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

packaging

-

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

immunohistochemistry: suitable (paraffin)

technique(s)

flow cytometry: 5.0-10.0 μg/mL, western blot: 0.1-0.2 μg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Insulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary agent in carbohydrate homeostasis, it has effects on fat metabolism.

Specificity

Recognizes insulin

Immunogen

Epitope: Insulin
Human recombinant Insulin

Application

Anti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P).
ELISA (Direct) Analysis:
Insulin-coated plate was incubated with Anti-Insulin, clone E11D7 in 2-fold serial dilutions diluted in PBST from 5 μg/mL to 0.01 μg/mL.
Research Category
Signaling

Metabolism
Research Sub Category
Insulin/Energy Signaling

Glucose/Glycogen Metabolism

Metabolic Hormones & Receptors

Quality

Immunohistochemistry Analysis:
20 μg/mL of this antibody detected insulin in β cells.

Target description

6 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monolconal IgG1κ in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05%NaN3

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Yoshimitsu Taguchi et al.
The Journal of biological chemistry, 291(23), 12029-12038 (2016-04-10)
Sphingosine-1-phosphate (S1P) is a sphingolipid metabolite that regulates basic cell functions through metabolic and signaling pathways. Intracellular metabolism of S1P is controlled, in part, by two homologous S1P phosphatases (SPPases), 1 and 2, which are encoded by the Sgpp1 and
Mi Ran Choi et al.
Journal of ginseng research, 41(4), 503-512 (2017-10-13)
Chronic heavy alcohol consumption may raise the risk of developing type 2 diabetes mellitus. Saponins inhibit apoptosis of pancreatic islet cells and reduce lipid parameters. The present study was designed to investigate the effect of saponin on chronic ethanol-treated diabetic
Gabriel Cao et al.
PloS one, 16(6), e0243340-e0243340 (2021-06-12)
The objective of this work was to analyze the structural changes of the pancreatic islets in rats, after 6 month consuming regular and light cola for 6 months. Also, we have analyzed the possible role of PDX-1 in that process.
Activation of PPAR? up-regulates the expression of insulin gene transcription factor MafA and ameliorates glucose-induced insulin secretion impaired by palmitate.
Mingming Cao,Yang Long,Yuzhen Tong,Jun Wan,Nanwei Tong
Molecular and Cellular Biochemistry null
Daniel Neumeier et al.
European journal of immunology, 52(2), 297-311 (2021-11-03)
Plasma cells and their secreted antibodies play a central role in the long-term protection against chronic viral infection. However, due to experimental limitations, a comprehensive description of linked genotypic, phenotypic, and antibody repertoire features of plasma cells (gene expression, clonal

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service