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Key Documents

05-117

Sigma-Aldrich

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

clone bFM-1, Upstate®, from mouse

Synonym(s):

Basic fibroblast growth factor, basic fibroblast growth factor bFGF, fibroblast growth factor 2, fibroblast growth factor 2 (basic), heparin-binding growth factor 2., GSK3 beta, GSK3B, Glycogen synthase kinase 3 beta, Glycogen synthase kinase 3β

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

bFM-1, monoclonal

form

liquid

mol wt

17.5 kDa

species reactivity

human, mouse, bovine, rat

manufacturer/tradename

Upstate®

technique(s)

neutralization: suitable
radioimmunoassay: suitable

isotype

IgG1κ

suitability

not suitable for Western blot

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

General description

Fibroblast growth factor 2 (FGF-2) belongs to the family of heparin-binding growth factors. It is localized in the cells of the mesoderm and neuroectodermal origin and several tumor cells. FGF-2 exists in four isoforms. It is made up of four cysteine residues, many basic residues, and two active sites. FGF-2 protein is a trigonal pyramidal structure formed by twelve anti-parallel β-sheets. The FGF-2 chromosome is located on the human chromosome at 4q28.1.

Research area: Cancer

Specificity

This antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.
This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.

Immunogen

Epitope: a.a. 5-24
Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.

Application

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 has been used in:

  • FGF2 neutralization assay (6 or 12 µg/mL)
  • in vitro tube formation assay (1000 ng/mL)
  • immunolabelling (1:200)

Biochem/physiol Actions

Fibroblast growth factor 2 (FGF-2) plays a role in vertebrate embryonic and fetal development. It acts as an inducer of angiogenesis and is a well-known mitogen (in vitro) for a variety of cells including fibroblasts and vascular endothelial cells. FGF-2 exerts its biological activity through high-affinity tyrosine kinase receptors and low-affinity heparan sulfate proteoglycans present on the cell surface. It exhibits autocrine effects on various cell functions involved in angiogenesis such as proliferation, integrin expression, migration, and proteinase production. FGF-2 participates in the growth, migration, differentiation, and survival of a variety of cells. The binding of FGF-2 with heparin protects FGF-2 from denaturation by heat and acid and proteolytic cleavage.

Quality

Routinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.

Neutralization of bFGF Activity Assay:
2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.

Target description

17.5 kDa

Physical form

Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Purified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Fetal liver or kidney tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Madalyn Hafenbreidel et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 40(13), 2907-2915 (2015-05-23)
Drug exposure results in structural and functional changes in brain regions that regulate reward and these changes may underlie the persistence of compulsive drug seeking and relapse. Neurotrophic factors, such as basic fibroblast growth factor (bFGF or FGF2), are necessary
T Korff et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(2), 447-457 (2001-02-07)
Paracrine interactions between endothelial cells (EC) and mural cells act as critical regulators of vessel wall assembly, vessel maturation and define a plasticity window for vascular remodeling. The present study was aimed at studying blood vessel maturation processes in a
Genome-wide meta-analysis implicates mediators of hair follicle development and morphogenesis in risk for severe acne
Petridis C, et al.
Nature Communications, 5075, 9(1)-9(1) (2018)
A novel mechanism of sequestering fibroblast growth factor 2 by glypican in lipid rafts, allowing skeletal muscle differentiation.
Jaime Gutierrez,Enrique Brandan
Molecular and cellular biology null
G Seghezzi et al.
The Journal of cell biology, 141(7), 1659-1673 (1998-07-01)
FGF-2 and VEGF are potent angiogenesis inducers in vivo and in vitro. Here we show that FGF-2 induces VEGF expression in vascular endothelial cells through autocrine and paracrine mechanisms. Addition of recombinant FGF-2 to cultured endothelial cells or upregulation of

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