mTOR (Mammalian Target of Rapamycin, FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 consists of at least mTOR, Raptor, and GL. mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The other mTOR complex, mTORC2, is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. TORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473. TORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

Other species to be tested.

This antibody only detects mTOR.

Full-length human mTOR

Confocal Immunofluorescent Immunocytochemistry:

Detect mTOR using this Anti-mTOR Antibody, clone 21A12.2 validated for use in WB & IC.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Evaluated by Western Blotting on HEK293 cell lysates.

Western Blotting Analysis: 1:1,000 - 2,000 dilution of this antibody was used to detect mTOR in HEK293 cell lysate.

~289 kDa

Format: Purified

Protein G Purified

Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% sodium azide.

Stable for 1 year at 2-8ºC from date of receipt.

Control
HEK293 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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