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Key Documents

05-172-AF488

Sigma-Aldrich

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor 488 Conjugate

clone Sm1.2, from mouse, ALEXA FLUOR 488

Synonym(s):

Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.45

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

Sm1.2, monoclonal

species reactivity

chicken, rat, mouse, human, fish

technique(s)

immunocytochemistry: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IGF1(3479)

General description

Insulin-like growth factor I (UniProt P05019; also known as IGF-I, Somatomedin-C) is encoded by the IGF1 (also known as IGFI, MGF) gene (Gene ID 3479) in human. Human insulin-like growth factor-I (IGF-I) is a single chain 70 amino acid polypeptide that shares structural homology with insulin and human IGF-II. IGF-I plays a critical role in growth, differentiation and development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-I is a regulator of metabolism and is also implicated in the pathophysiology of cancer, diabetes and obesity. Upon binding the tetrameric (alpha:beta)2 IGF-I receptor, the receptor alpha chains induce the tyrosine autophosphorylation of the beta chains, which in turn trigger cell type-specific downstream signaling cascade of events.

Specificity

IGF-I (Russel, W.E., (1984). Proc. Natl. Acad. Sci. U. S. A. 81(8):2389-2392). 40% cross reactivity to IGF-II (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522).

Immunogen

Highly purified human plasma insulin-like growth factor I (IGF-I; Somatomedin C; Sm-C) conjugated to mouse albumin (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522). Action of somatomedins on cell growth: Effect of selective neutralisation of somatomedin C (IGF-I) with a monoclonal antibody. In: Raiti, S., Tolman, and R.A. (eds.), Human Growth Hormone. pp. 585–599. Plenum Publishing Corp., New York, NY).

Application

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor 488 Conjugate is an antibody against IGF-I for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IGF-I in HeLa and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-172) is shown to be suitable also for Western blotting, immunohistochemistry, immunoprecipitation, and neutralization applications.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IGF-I in A431 cells.

Target description

~17 kDa observed

Physical form

Protein G Purified
Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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