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05-545

Sigma-Aldrich

Anti-PP2A Antibody, C subunit, clone 7A6

culture supernatant, clone 7A6, Upstate®

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Synonym(s):
Anti-NEDLBA, Anti-PP2CA, Anti-PP2Calpha, Anti-RP-C
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

7A6, monoclonal

species reactivity

mouse, Saccharomyces cerevisiae, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PPP2CA(5515)

Specificity

Recognizes the catalytic subunit of PP2A.

Immunogen

peptide (C-bARRTPDYFL) corresponding to amino acids 302-309 of human PP2A catalytic subunit

Application

Anti-PP2A Antibody, C subunit, clone 7A6 detects level of PP2A & has been published & validated for use in IP & WB.
Research Category
Signaling

Neuroscience
Research Sub Category
Kinases & Phosphatases

Neurodegenerative Diseases

Quality

routinely evaluated by immunoblot on RIPA lysates from A431 cells

Target description

36 kDa

Physical form

Cell culture supernatant containing 30% glycerol and 0.05% sodium azide as a preservative.
Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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