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05-637-AF488

Sigma-Aldrich

Anti-Bmi-1 Antibody, clone F6, Alexa Fluor 488

clone F6, from mouse, ALEXA FLUOR 488

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Synonym(s):
Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, Bmi-1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

F6, monoclonal

species reactivity

human, monkey

species reactivity (predicted by homology)

rabbit (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BMI1(648)

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This Item
05-63705-1321SAB4200034
vibrant-m

05-637

Anti-Bmi-1 Antibody, clone F6

vibrant-m

05-1321

Anti-BMI1 Antibody, clone AF27

clone

F6, monoclonal

clone

F6, monoclonal

clone

AF27, monoclonal

clone

polyclonal

conjugate

ALEXA FLUOR 488

conjugate

-

conjugate

-

conjugate

unconjugated

species reactivity

human, monkey

species reactivity

rat, human, mouse, rabbit

species reactivity

human

species reactivity

human

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

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General description

Polycomb complex protein BMI-1 (UniProt P25916; also known as B lymphoma Mo-MLV insertion region 1, Polycomb group RING finger protein 4) is encoded by the Bmi1 (also known as AW546694, Bmi-1, Pcgf4) gene (Gene ID 12151) in murine species. Bmi1 is a member of the polycomb group (PcG) family of evolutionarily conserved gene silencers involved in cell fate decisions and body pattern during development. Bmi1 is an important regulator of self-renewal of hematopoietic and neural stem cells, as well as cancer stem cells (CSCs). Bmi1 is a main constituent of the polycomb repressive complex 1 (PRC1) and pariticipates in PRC-mediated gene silencing by promoting monoubiquitination of H2A. Bmi1 overexpression is reported in many different human cancers and high level of Bmi1 is considered as a poor prognostic marker. Bmi1 targets genes involved in transforming growth factor-β/bone morphogenetic protein (TGF-beta/BMP), endoplasmic reticulum (ER) stress, and WNT pathways. Bmi itself is transcriptionally regulated by c-Myc and E2F1 transcription factors.

Immunogen

Epitope: a.a. 1-202
Recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
The unconjugated version (Cat. No. 05-637) is also shown to be suitable for Western blotting, Immunocytochemistry, and Immunohistochemistry applications.
This Anti-Bmi-1 Antibody, clone F6, Alexa Fluor 488 is validated for use in Immunocytochemistry for the detection of Bmi-1.

Quality

Evaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HepG2 cells.

Target description

40-44 kDa observed

Physical form

Protein G Purified
Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Violette Azzoni et al.
Cell death & disease, 13(2), 96-96 (2022-02-04)
Replication stress (RS) has a pivotal role in tumor initiation, progression, or therapeutic resistance. In this study, we depicted the mechanism of breast cancer stem cells' (bCSCs) response to RS and its clinical implication. We demonstrated that bCSCs present a

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