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05-689

Sigma-Aldrich

Anti-HP1α Antibody, clone15.19s2

clone 15.19s2, Upstate®, from mouse

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Synonym(s):
Antigen p25, HP1 alpha, HP1Hs alpha, Heterochromatin protein 1 homolog alpha, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), heterochromatin protein 1-alpha
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

15.19s2, monoclonal

species reactivity

mouse, vertebrates, human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CBX5(23468)

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Anti-HP1α Antibody, clone15.19s2 clone 15.19s2, Upstate®, from mouse

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Anti-HP1α Antibody, clone15.19s2

Anti-HP1γ Antibody, clone 42s2 clone 42s2, Upstate®, from mouse

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Anti-HP1γ Antibody, clone 42s2

Anti-HP1γ Antibody, clone 14D3.1 clone 14D3.1, from mouse

MABE656

Anti-HP1γ Antibody, clone 14D3.1

species reactivity

mouse, vertebrates, human

species reactivity

human, mouse

species reactivity

human

species reactivity

human

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

wet ice

shipped in

dry ice

shipped in

ambient

shipped in

ambient

technique(s)

ChIP: suitable, immunohistochemistry: suitable, western blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

ChIP: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

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General description

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation.

Specificity

This antibody recognizes HP1α

Immunogen

GST fusion protein corresponding to residues 2-191 of human HP1α protein.

Application

Anti-HP1α Antibody, clone15.19s2 is a Mouse Monoclonal Antibody for detection of HP1α also known as HP1Hs alpha, Heterochromatin protein 1 homolog alpha & has been validated in ChIP, ICC, IHC, IP & WB.
Immunohistochemistry:
A previous lot of this antibody has been reported by an independent laboratory to detect HP1a in NIH/3T3 nuclei (Ayyanathan, K., 2003).

Immunoprecipitation/Chromatin Immunoprecipitation:
A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate HP1α from nuclear extracts and formalin-cross-linked chromatin (Ayyanathan, K., 2003).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

RNA Binding Protein (RBP)

Quality

Routinely evaluated by western blot in acid-extracted HeLa cell lysates.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected HP1α protein in acid-extracted HeLa cell lysates.

Target description

29 kDa

Linkage

Replaces: MABE479

Physical form

Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% Sodium Azide before the addition of glycerol to 30%.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Analysis Note

Control
HeLa, NIH/3T3 and COS cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Due to license agreement restrictions, this product cannot be purchased for resale.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Histone modification in constitutive heterochromatin versus unexpressed euchromatin in human cells.
Guanchao Jiang, Fan Yang, Cecilia Sanchez, Melanie Ehrlich
Journal of Cellular Biochemistry null
Eva Bártová et al.
Differentiation; research in biological diversity, 76(1), 24-32 (2007-06-19)
Human embryonic stem cells (hES) are unique in their pluripotency and capacity for self-renewal. Therefore, we have studied the differences in the level of chromatin condensation in pluripotent and all-trans retinoic acid-differentiated hES cells. Nuclear patterns of the Oct4 (6p21.33)
Differentiation-independent fluctuation of pluripotency-related transcription factors and other epigenetic markers in embryonic stem cell colonies.
Sustackova, G; Legartova, S; Kozubek, S; Stixova, L; Pachernik, J; Bartova, E
Stem Cells and Development null
Loss of heterochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell cycle progression in mice.
Abe, K; Naruse, C; Kato, T; Nishiuchi, T; Saitou, M; Asano, M
Biology of reproduction null
Fangwei Wang et al.
Molecular and cellular biology, 26(11), 4028-4040 (2006-05-18)
A role for the RNA interference (RNAi) pathway in the establishment of heterochromatin is now well accepted for various organisms. Less is known about its relevance and precise role in mammalian cells. We previously showed that tandem insertion of a

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