MilliporeSigma
All Photos(1)

Documents

05-902R

Sigma-Aldrich

Anti-HA Tag Antibody, clone 114-2C-7, rabbit monoclonal

clone 114-2C-7, Upstate®, from rabbit

Sign Into View Organizational & Contract Pricing

eCl@ss:
32160702
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

114-2C-7, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

unmodified

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
04-90205-904SAB4300603
Anti-HA Tag Antibody Upstate®, from mouse

05-904

Anti-HA Tag Antibody

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

clone

114-2C-7, monoclonal

clone

DW2, monoclonal

clone

monoclonal

clone

polyclonal

isotype

IgG

isotype

IgG

isotype

IgG3

isotype

IgG

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: 1:500-1:1000

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. 05-902R is a recombinant version of the rabbit monoclonal Cat no. 05-902.

Specificity

The antibody recognizes recombinant proteins containing the HA epitope tag.

Immunogen

Synthetic peptide containing the sequence ...CYPYDVPDY... derived from the influenza virus hemaglutinin (HA) protein.

Application

Anti-HA Tag Antibody, clone 114-2C-7 detects level of HA Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

Routinely evaluated by Western Blot HA-tagged WNT-1 transfected HEK293 cells.

Physical form

Format: Purified
Protein A purified
Protein A purified rabbit immunoglobulin in buffer containing 60mM Tris, 90 mM NaCl, 0.03% NaN3, 60mM glycine, pH 7.5, and 40% glycerol.

Storage and Stability

Maintain for 1 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Manuel Osorio-Valeriano et al.
Molecular cell, 81(19), 3992-4007 (2021-09-26)
ParB-like CTPases mediate the segregation of bacterial chromosomes and low-copy number plasmids. They act as DNA-sliding clamps that are loaded at parS motifs in the centromere of target DNA molecules and spread laterally to form large nucleoprotein complexes serving as
Dario Zimmerli et al.
eLife, 9 (2020-08-19)
BCL9 and PYGO are β-catenin cofactors that enhance the transcription of Wnt target genes. They have been proposed as therapeutic targets to diminish Wnt signaling output in intestinal malignancies. Here we find that, in colorectal cancer cells and in developing
Rongkun Li et al.
Molecular cancer, 18(1), 18-18 (2019-02-02)
Lysyl oxidase-like 4 (LOXL4) has been found to be dysregulated in several human malignancies, including hepatocellular carcinoma (HCC). However, the role of LOXL4 in HCC progression remains largely unclear. In this study, we investigated the clinical significance and biological involvement
Meng-Ling Wu et al.
Cell communication and signaling : CCS, 12, 22-22 (2014-03-29)
Vascular smooth muscle cells (VSMCs) of the arterial wall play a critical role in the development of occlusive vascular diseases. Cysteine-rich protein 2 (CRP2) is a VSMC-expressed LIM-only protein, which functionally limits VSMC migration and protects against pathological vascular remodeling.
Anna Nordin et al.
Genome biology, 24(1), 185-185 (2023-08-11)
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an increasingly popular technique to map genome-wide binding profiles of histone modifications, transcription factors, and co-factors. The ENCODE project and others have compiled blacklists for ChIP-seq which have been widely adopted:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service