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06-229

Sigma-Aldrich

Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody

Upstate®, from rabbit

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Synonym(s):
CD340 antigen, MLN 19, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, neuroblastoma/glioblastoma derived oncogene homolog, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma der
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

packaging

antibody small pack of 25 μL

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

phosphorylation (pTyr1248)

Gene Information

human ... ERBB2(2064)

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This Item
06-56205-39009-310
vibrant-m

06-562

Anti-erbB-2/HER-2 Antibody

species reactivity

human, rat

species reactivity

monkey, human, mouse

species reactivity

human, bovine, mouse, rat

species reactivity

human

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

2F12, monoclonal

clone

polyclonal

isotype

IgG

isotype

IgG

isotype

IgG2aκ

isotype

-

packaging

antibody small pack of 25 μL

packaging

-

packaging

-

packaging

-

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General description

The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.

Specificity

Predicted to cross react with rat based on conservation of immunogenic sequence.
Recognizes and is specific for the activated form of erbB-2/HER-2 oncoprotein, MW ~138 kDa.

Immunogen

Epitope: Tyr1248
Synthetic phosphopeptide, residues 1243-1255, corresponding to a major auto-phosphorylation (Tyr1248) site of human erbB-2/HER-2 (C-AENPEpYLGLDVPV).

application

Immunohistochemistry:
IHC was performed by an independent laboratory.

Immunocytochemistry:
2 µg/mL of a previous lot showed positive immunostaining for phospho-erbB2/HER-2 in EGF treated A431 cells.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Kinases & Phosphatases
This Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody is validated for use in IC, WB for the detection of phospho-erbB-2/HER-2 (Tyr1248).

Quality

Evaluated by Western Blot on A431+/-EGF lysates.

Western Blotting Analysis:
1:1000 dilution of this antibody detected phospho-erbB2/Her2 on 10 µg of A431+EGF lysates.

Target description

185 kDa

Physical form

Double immunoaffinity purified rabbit polyclonal IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4
ImmunoAffinity Purified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
EGF treated A431 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yuichi Abe et al.
Neuroscience research, 63(2), 138-148 (2008-12-17)
Ligands for the epidermal growth factor receptor ErbB1, such as epidermal growth factor (EGF) and transforming growth factor alpha (TGFalpha), negatively regulate synaptic maturation of GABAergic neurons in the developing neocortex. Here, we evaluated the effects of these ligands in
BIBX1382BS, but not AG1478 or PD153035, inhibits the ErbB kinases at different concentrations in intact cells.
M Egeblad, O H Mortensen, L C van Kempen, M Jaattela
Biochemical and biophysical research communications null
Activation of ErbB2 during wallerian degeneration of sciatic nerve.
Kwon, Y K, et al.
The Journal of Neuroscience, 17, 8293-8299 (1997)
Tonghui Wang et al.
Cancer science, 108(4), 671-677 (2017-02-07)
It is well documented that human epidermal growth factor receptor 2 (HER2) overexpression/amplification is associated with poor survival in breast cancer patients. However, it is largely unknown whether HER2 somatic mutations are associated with survival in HER2-negative breast cancer patients.
Bay846, a new irreversible small molecule inhibitor of EGFR and Her2, is highly effective against malignant brain tumor models.
Longo, Sharon L, et al.
Investigational New Drugs, 30, 2161-2172 (2012)

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