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Key Documents

06-238

Sigma-Aldrich

Anti-Gβ Antibody

serum, Upstate®

Synonym(s):

Anti-HG2C1, Anti-NEDHYDF, Anti-SSS4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GNB2(2783)

Specificity

Gβ subunits; recognizes both isoforms

Immunogen

Internal sequence of G-protein β subunit (KTREGNVRVSREL) conjugated to octavalent polylysine base

Application

Detect Gβ using this Anti-Gβ Antibody validated for use in IP & WB.

Quality

Immunoblot Analysis: A 1:500-1:1000 dilution of this antibody detected the Gβ subunits in 20 μg of a cell lysate prepared from rat brain microsomal preparation (Catalog # 12-144).

Immunoprecipitation/Immunoblot: 10 μl of this antibody immunoprecipitated the Gβ subunit from 500 μg-1mg of a lysate of rat brain microsomal preparation (Catalog # 12-144).

Target description

35/36kDa

Physical form

PBS, pH 7.2

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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J Yamauchi et al.
The Journal of biological chemistry, 272(12), 7602-7607 (1997-03-21)
G protein beta and gamma subunits (Gbeta and Ggamma) form a complex that is involved in various signaling pathways. We reported that the C-terminal 10 amino acids of Gbeta are required for association with Ggamma (Yamauchi, J., Kaziro, Y., and
Activation of p38 mitogen-activated protein kinase (p38 MAPK), extracellular signal-regulated kinase (ERK) and c-jun N-terminal kinase (JNK) during hypoxia in cerebral cortical nuclei of guinea pig fetus at term: role of nitric oxide.
Dev Maulik, Qazi M Ashraf, Om P Mishra, Maria Delivoria-Papadopoulos
Neuroscience Letters null
J Yamauchi et al.
The Journal of biological chemistry, 274(4), 1957-1965 (1999-01-16)
Heterotrimeric G protein beta gamma subunit (Gbeta gamma) mediates signals to two types of stress-activated protein kinases, c-Jun NH2-terminal kinase (JNK) and p38 mitogen-activated protein kinase, in mammalian cells. To investigate the signaling mechanism whereby Gbeta gamma regulates the activity
J Yamauchi et al.
The Journal of biological chemistry, 272(44), 27771-27777 (1997-11-05)
Various extracellular stimuli activate three classes of mitogen-activated protein kinases (MAPKs): extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 MAPK. In mammalian cells, p38 MAPK is activated by endotoxins, inflammatory cytokines, and environmental stresses. We show here that p38 MAPK
M C Galas et al.
The Journal of biological chemistry, 272(5), 2788-2793 (1997-01-31)
The ADP-ribosylation factor (ARF) GTP-binding proteins are believed to function as regulators of vesicular budding and fusion along the secretory pathway. To investigate the role of ARF in regulated exocytosis, we have examined its intracellular distribution in cultured chromaffin cells

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