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06-284-AF488

Sigma-Aldrich

Anti-Nitrotyrosine Antibody Alexa Fluor 488 Conjugate

from rabbit, ALEXA FLUOR 488

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Synonym(s):
Nitrotyrosine AlexaFluor 488 Conjugate
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

all

technique(s)

immunocytochemistry: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

nitration (Tyr)

Gene Information

human ... NOS1(4842)

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06-284-AF64716-226AB5320A4
conjugate

ALEXA FLUOR 488

conjugate

ALEXA FLUOR 647

conjugate

ALEXA FLUOR 488

conjugate

ALEXA FLUOR 488

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

clone

polyclonal

clone

polyclonal

clone

1A6, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

vertebrates

species reactivity

rat

technique(s)

immunocytochemistry: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

flow cytometry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

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General description

Nitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration. In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.

Specificity

This rabbit polyclonal antibody detects tyrosine nitrated proteins.

Immunogen

Epitope: Nitrated Tyrosine
Nitrated Keyhole Limpet hemocyanain (KLH).

Application

Anti-Nitrotyrosine AlexaFluor 488 Conjugate, Cat. No. 06-284-AF488, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.
Research Category
Signaling
Research Sub Category
Developmental Signaling
The unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.

Quality

Evaluated by Immunocytochemistry in A431 cells treated with peroxytnitrite.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.

Physical form

Affinity purified
Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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