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06-503-I

Sigma-Aldrich

Anti-Caspase 1 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Caspase-1, CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CASP1(834)

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This Item
SAB4503272SAB5700660PRS3463
Anti-Caspase 1 Antibody from rabbit, purified by affinity chromatography

06-503-I

Anti-Caspase 1 Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

-

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

rat, human, mouse

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: 1:10000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200, immunoprecipitation (IP): 1:50-1:100, western blot: 1:500-1:2000

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

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General description

Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an enzyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain.

Specificity

Other homologies: Mouse, rat, canine, porcine and feline. (92% sequence homology).
This antibody recognizes the p20 subunit of Caspase 1. This antibody may cross-react with Caspase 5 due to 92% sequence homology. Additionally, this antibody recognizes the P45 proenzyme (~45 kDa) and the p20 subunit (~20 kDa) of the reduced, human Caspase 1 isoforms.

Immunogen

Epitope: p20 subunit
KLH-conjugated linear peptide corresponding to the p20 subunit of human Caspase 1.

Application

Anti-Caspase 1 Antibody is a highly specific rabbit polyclonal antibody, that targets Caspase 1 & has been tested in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Caspase 1 in human spleen tissue. Additionally, a 1:4,000 dilution from a representative lot detected Caspase 1 in human colon tissue.
Research Sub Category
Caspases

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 5 µg/mL of this antibody detected Caspase 1 in 10 µg THP-1 cell lysate.

Target description

~45 kDa observed. Five isoforms between 10-45 kDa are known to exist. This antibody recognizes the P45 proenzyme (~45 kDa) and the p20 subunit (~20 kDa) of the reduced, human Caspase 1 isoforms.

Linkage

Replaces: 06-503

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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