Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.

Detects phosphorylated Smad3 (Ser423 and Ser425).

Epitope: Phosphorylated Ser423/425

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser423/425 of human Smad3.

Anti-phospho-Smad3 (Ser423/425) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Smad3 (Ser423/425) also known as MAD Mothers against DPP homolog 3 & has been validated in WB.

Research Category
Signaling

Evaluated by western blot on untreated and UV-treated AKR-2B cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect Smad3 in UV-treated AKR-2B cell lysate.

50 kDa

Unpurified rabbit serum in buffer containing 0.05% sodium azide.

Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

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