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07-1492

Sigma-Aldrich

Anti-Calcineurin Aα Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
NFAT transcription complex cytosolic component, nuclear factor of activated T-cells, cytoplasmic,, calcineurin-dependent 1, nuclear factor of activated T-cells, cytosolic component 1
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse, human

species reactivity (predicted by homology)

mammals (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PPP3CA(5530)
mouse ... Ppp3Ca(19055)
rat ... Ppp3Ca(24674)

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04-804ABS403HPA008789
vibrant-m

07-1492

Anti-Calcineurin Aα Antibody

vibrant-m

04-804

Anti-NFAT1 Antibody, clone 8G4.2

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

species reactivity

rat, mouse, human

species reactivity

rat, mouse, human

species reactivity

human

species reactivity

human

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

NCBI accession no.

NP_000935.1

NCBI accession no.

NP_001129493.1

NCBI accession no.

NP_068810

NCBI accession no.

-

Gene Information

human ... PPP3CA(5530)
mouse ... Ppp3Ca(19055)
rat ... Ppp3Ca(24674)

Gene Information

human ... NFATC2(4773)

Gene Information

human ... RELA(5970)

Gene Information

human ... NFATC2(4773)

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General description

Calcineurin is a serine/threonine protein phosphatase involved in the mediation of signal transduction in a wide variety of cellular responses. Calcineurin has been linked to Pmk1 MAP kinase and PI pathways, as well as to members of the small GTPase Rab/Ypt family and Type II myosin. Furthermore, calcineurin participates in the transduction of extracellular signals to the nucleus by dephosphorylation of members of the NFAT family of transcription factors. These linkages demonstrate calcineurin′s multifunctional capabilities in the regulation of calcium signaling. The Aα form of calcineurin is a major catalytic subunit gene isoform of calcineurin in most tissues that allows differentiation of Aα from the Aβ and Aγ isoforms.

Specificity

This antibody recognizes the Aα form of calcineurin.

Immunogen

KLH-conjugagted synthetic peptide corresponding to Calcineurin Aα.

Application

Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Calcineurin Aα Antibody is validated for use in WB for the detection of Calcineurin Aα.

Quality

Evaluated by Western Blot in Mouse brain lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calcineurin on 10 µg of mouse brain lysate.

Target description

58 kDa

Linkage

Replaces: AB1696

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 40% gylerol. Contains no preservative.

Storage and Stability

Store at -20°C for up to 12 months in undiluted aliquots; at this temperature the solution will
remain unfrozen.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Mouse brain lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Benjamin M Zemel et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(34), 8256-8272 (2017-07-29)
Dysfunction of the fast-inactivating Kv3.4 potassium current in dorsal root ganglion (DRG) neurons contributes to the hyperexcitability associated with persistent pain induced by spinal cord injury (SCI). However, the underlying mechanism is not known. In light of our previous work
Pradoldej Sompol et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(25), 6132-6148 (2017-06-01)
Hyperexcitable neuronal networks are mechanistically linked to the pathologic and clinical features of Alzheimer's disease (AD). Astrocytes are a primary defense against hyperexcitability, but their functional phenotype during AD is poorly understood. Here, we found that activated astrocytes in the
Calcineurin regulates nuclear factor I dephosphorylation and activity in malignant glioma cell lines.
Brun, M; Glubrecht, DD; Baksh, S; Godbout, R
The Journal of Biological Chemistry null
Jennifer L Furman et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(5), 1502-1515 (2016-02-05)
Increasing evidence suggests that the calcineurin (CN)-dependent transcription factor NFAT (Nuclear Factor of Activated T cells) mediates deleterious effects of astrocytes in progressive neurodegenerative conditions. However, the impact of astrocytic CN/NFAT signaling on neural function/recovery after acute injury has not
Roberto Mota et al.
The American journal of pathology, 188(7), 1676-1692 (2018-05-15)
The muscle-specific ubiquitin ligase atrogin-1 (MAFbx) has been identified as a critical regulator of pathologic and physiological cardiac hypertrophy; it regulates these processes by ubiquitinating transcription factors [nuclear factor of activated T-cells and forkhead box O (FoxO) 1/3]. However, the

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