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07-571-I

Sigma-Aldrich

Anti-nNOS/NOS I Antibody

0.5 mg/mL, from rabbit

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Synonym(s):
Nitric oxide synthase, brain, bNOS, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Peptidyl-cysteine S-nitrosylase NOS1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

concentration

0.5 mg/mL

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Nos1(18125)
rat ... Nos1(24598)

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This Item
07-428-I07-520ABN26
Anti-nNOS/NOS I Antibody 0.5 mg/mL, from rabbit

07-571-I

Anti-nNOS/NOS I Antibody

Anti-eNOS/NOS III Antibody, CT Upstate®, from rabbit

07-520

Anti-eNOS/NOS III Antibody, CT

Anti-iNOS/NOS II Antibody, NT from rabbit, purified by affinity chromatography

ABN26

Anti-iNOS/NOS II Antibody, NT

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

species reactivity

mouse, rat

species reactivity

human

species reactivity

mouse, pig, guinea pig, rat, canine, human, bovine

species reactivity

mouse, human

UniProt accession no.

P29476

UniProt accession no.

P29474

UniProt accession no.

P29474

UniProt accession no.

P29477

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

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General description

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. nNOS/NOS I, also known as NC-NOS, or NOS type I, or Neuronal NOS, or Peptidyl-cysteine S-nitrosylase NOS1 and bNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NOS I has been suggested to have nitrosylase activity and to mediate cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Immunogen

Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to the near N-terminus of rat nNOS/NOS I.

Application

Anti-nNOS/NOS I Antibody is an antibody against nNOS/NOS I for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected nNOS/NOS I in rat cerebellum and cerebral cortex tissues, and mouse hippocampus and thalamus tissues.
Research Category
Apoptosis & Cancer
Research Sub Category
Neurodegenerative Diseases

Quality

Evaluated by Western Blotting in rat brain microsomal preparation.

Western Blotting Analysis: A 1:500 dilution of this antibody detected nNOS/NOS I in 10 µg of rat brain microsomal preparation.

Target description

~165 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Linkage

Replaces: 07-571

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Arina Ponomarenko et al.
Marine drugs, 20(8) (2022-08-26)
At present, the study of the neurotropic activity of polyunsaturated fatty acid ethanolamides (N-acylethanolamines) is becoming increasingly important. N-docosahexaenoylethanolamine (synaptamide, DHEA) is a highly active metabolite of docosahexaenoic acid (DHA) with neuroprotective, synaptogenic, neuritogenic, and anti-inflammatory properties in the nervous
Shao-Rui Chen et al.
Neuropharmacology, 125, 156-165 (2017-07-30)
The role of nitric oxide (NO) in nociceptive transmission at the spinal cord level remains uncertain. Increased activity of spinal N-methyl-d-aspartate (NMDA) receptors contributes to development of chronic pain induced by peripheral nerve injury. In this study, we determined how

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