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09-272

Sigma-Aldrich

Anti-phospho-p38α (Thr180/Tyr182) Antibody

Upstate®, from rabbit

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Synonym(s):
SAPK2a, MAPK14, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, p38ALPHA, mitogen-activated protein kinase 14
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

packaging

antibody small pack of 25 μL

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pThr180/pTyr182)

Gene Information

human ... MAPK14(1432)

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This Item
05-105907-626MABS64
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

antibody form

affinity isolated antibody

antibody form

culture supernatant

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

polyclonal

clone

8.78.8, monoclonal

clone

polyclonal

clone

6E5.2, monoclonal

UniProt accession no.

Q16539

UniProt accession no.

Q15759

UniProt accession no.

Q13131

UniProt accession no.

Q16539

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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Specificity

Recognizes p38α dual phosphorylation of Thr180 and Tyr182.

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-p38α (Thr180/Tyr182).

Application

Immunoblot Analysis: A 1:1,000 dilution of the lot detected p38α dually phosphorylated on residues Thr180 and Tyr182 in lysate from HeLa cells stimulated with anisomycin.

Immunfluorescence: A 1:100 dilution of this antibody detected p38α phosphorylated on both residues, Thr180 and Tyr182, in fixed HeLa cells stimulated with anisomycin.

Immunoprecipitation (IP): 2-5 μL (5-10 μL/mL) of this antibody immunoprecipitated both active p38α from anisomycin treated HeLa whole cell extract and human recombinant GST-p38α, separately, in RIPA buffer.

Immunoprecipitation Kinase Reaction (IPK): 2-5 μL of this lot immunoprecipitated phosphorylated p38α from both anisomycin treated HeLa cells as well as recombinant p38α, separately, and then placed into an in vitro Kinase reaction with recombinant GST-ATF-2 where it phosphorylated ATF-2 on Thr69/71.
Research Category
Signaling
Research Sub Category
MAP Kinases
This Anti-phospho-p38α (Thr180/Tyr182) Antibody is validated for use in IP, WB for the detection of phospho-p38α (Thr180/Tyr182).

Quality

Routinely evaluated by immunoblot.

Target description

38 kDa

Linkage

Replaces: AB3828

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Anisomycin treated HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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