MilliporeSigma
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1.00908

Millipore

Readybag® Buffered Peptone Water

According to ISO 6579, ISO 21528, ISO 22964, FDA-BAM, APHA, and EP; For microbiology

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Synonym(s):
Tryptone Phosphate Water, Peptone Water (buffered), BPW

Agency

APHA
EP
ISO 21528
ISO 22964
ISO 6579

Quality Level

reg. compliance

FDA BAM

sterility

sterile; γ-irradiated

form

medium granules (dehydrated (DCM))

manufacturer/tradename

ReadyBag®

technique(s)

microbiological culture: suitable

pH

7.2 (37 °C, 25 g/L in H2O, after autoclaving)

solubility

25.5 g/L

bulk density

800 kg/m3

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Cronobacter spp.
Salmonella spp.
enterobacteriaceae

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This Item
1.0090101202141.46142
Buffered Peptone Water according to ISO 6579, ISO 21528, ISO 22964, FDA-BAM, EP, sterile; γ-irradiated, ReadyBag®

Millipore

1.00901

Buffered Peptone Water

sterility

sterile; γ-irradiated

sterility

sterile; γ-irradiated

sterility

sterile

sterility

-

form

medium granules (dehydrated (DCM))

form

medium granules

form

liquid

form

liquid (ready-to-use)

manufacturer/tradename

ReadyBag®

manufacturer/tradename

ReadyBag®

manufacturer/tradename

-

manufacturer/tradename

ReadyTube®

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

pH

7.2 (37 °C, 25 g/L in H2O, after autoclaving)

pH

7.2 (37 °C, 25 g/L in H2O)

pH

-

pH

7.0 ( in H2O, ready-to-use solution)

General description

Buffered Peptone water is a nutrient broth media that aids in resuscitating sub-lethally injured bacteria and enables intense growth. The phosphate buffer system prevents bacterial damage caused by changes in the pH of the medium. Peptone, including enzymatic digest of casein acts as a source of carbon, nitrogen, vitamins, and minerals whilst sodium chloride maintains the osmotic balance. The Readybag® sachets are gamma-irradiated (10-20 kGray). Each Readybag® sachet contains 86 g of granulated Buffered Peptone Water broth, which can be reconstituted on addition of sterile water.

Application

Readybag® Buffered Peptone Water is suitable for the preliminary non-selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae, such as Salmonella and Cronobacter, from food and animal feed, water, and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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Protocols

New EN ISO 22964 for Cronobacter spp. detection in the food chain

The new EN ISO 22964:2017 standard specifies a horizontal method for the detection of Cronobacter spp. in food, animal feed and environmental samples.

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