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Millipore

Readybag® Half Fraser Broth with Supplements

acc. ISO 11290, 62 g, irradiated, For the primary selective enrichment of Listeria sp.

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Synonym(s):
Demi Fraser broth, Fraser broth primary enrichment, Fraser Broth, Fraser Listeria selective enrichment Broth

Agency

APHA
ISO 11133
ISO 11290

Quality Level

form

medium granules (dehydrated (DCM))

manufacturer/tradename

ReadyBag®

technique(s)

microbiological culture: suitable

pH

7.0-7.4 (25 °C, 55 g/L in H2O)

solubility

55 g/L

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Listeria spp.

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01202161.401971.00025
form

medium granules (dehydrated (DCM))

form

liquid

form

-

form

medium granules (dehydrated (DCM))

manufacturer/tradename

ReadyBag®

manufacturer/tradename

-

manufacturer/tradename

EcoCult®

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

pH

7.0-7.4 (25 °C, 55 g/L in H2O)

pH

-

pH

-

pH

7.0-7.4 (25 °C, 55 g/L in H2O, after autoclaving)

solubility

55 g/L

solubility

-

solubility

-

solubility

55 g/L

General description

Half FRASER (Demi FRASER) broth has high nutrient content and the large buffer capacity that creates optimum growth conditions for Listeria. Lithium chloride, nalidixic acid, and acriflavine hydrochloride largely inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate enables the detection of the β-D-glucosidase activity by the formation of a black complex of esculetin with iron (III) ions. However, this reaction is not exclusive to Listeria sp. and hence every primary and secondary enrichment in FRASER broth has to be sub-cultured on selective plating media.

Application

Half FRASER (Demi FRASER) broth with supplements is used for the primary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Types of Media in Microbiology

The different types of culture media, that are used to grow microorganisms in the laboratory for quality control, are classified by several criteria, such as consistency, composition, or selectivity.

Protocols

Listeria: a Survivor

In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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