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1.15933

Sigma-Aldrich

Carmine (C.I. 75470)

(calcium-aluminium lacquer of carminic acid) for microscopy Certistain®

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Synonym(s):
Carmine (C.I. 75470)
Empirical Formula (Hill Notation):
C44H37AlCaO27 · 3H2O
CAS Number:
Molecular Weight:
1118.85
EC Index Number:
215-724-4

Quality Level

form

solid

composition

Dye, ≥50% spectrophotometric assay

solubility

practically insoluble 1.3 g/L

bulk density

290 kg/m3

εmax

105-150 at 525-532 nm in DMSO at 0.05 g/L
80-110 at 565-570 nm in DMSO at 0.05 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C22H20O13/c1-4-8-5(2-6(24)9(4)22(33)34)13(25)10-11(15(8)27)16(28)12(18(30)17(10)29)21-20(32)19(31)14(26)7(3-23)35-21/h2,7,14,19-21,23-24,26,28-32H,3H2,1H3,(H,33,34)

InChI key

DGQLVPJVXFOQEV-UHFFFAOYSA-N

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This Item
1.042111.159381.15939
Carmine (C.I. 75470) (calcium-aluminium lacquer of carminic acid) for microscopy Certistain®

1.15933

Carmine (C.I. 75470)

Sigma-Aldrich

1.04211

Carminic acid (C.I. 75470)

Nuclear fast red (C.I. 60760) for microscopy Certistain®

1.15939

Nuclear fast red (C.I. 60760)

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

practically insoluble 1.3 g/L

solubility

30 g/L

solubility

slightly soluble at 20 °C

solubility

1 g/L

εmax

105-150 at 525-532 nm in DMSO at 0.05 g/L, 80-110 at 565-570 nm in DMSO at 0.05 g/L

εmax

-

εmax

155-165 at 290-292 nm in acetonitrile at 0.05 g/L

εmax

380-422 at 557-559 nm in DMSO at 0.02 g/L, 383-426 at 520-522 nm in DMSO at 0.02 g/L

Quality Level

400

Quality Level

200

Quality Level

400

Quality Level

400

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

General description

Carmine (C.I. 75470) (calcium-aluminium lacquer of carminic acid) for microscopy Certistain®, is a dry dye that is used for the preparation of a staining solution for staining nuclei and for demonstration of glycogen in histological sections of human origin. As a Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Identity
- aluminium:
passes test
- Calcium: passes test
- UV/VIS-Spectrum: passes test
Dye content (spectrophotometrically): ≥ 50 % (als C₂₂H₂₀O₁₃)
Absorption maximum λ 1 (Dimethyl sulfoxide): 565 - 570 nm
Absorption maximum λ 2 (Dimethyl sulfoxide): 525 - 532 nm
Spec. Absorptivity A 1%/1cm
- λ1max; 0.05 g/l; DMSO:
80 - 110
- λ2max; 0.05 g/l; DMSO: 105 - 150
TLC-Test: passes test
Water (according to Karl Fischer): ≤ 15 %
Sulfated ash: 10 - 17 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_c

Not applicable


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