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1.16434

Millipore

Yersinia selective agar (base) acc. to SCHIEMANN (CIN-agar)

for the selective cultivation of Yersinia

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Quality Level

form

powder (dehydrated (DCM))

technique(s)

microbiological culture: suitable

pH

7.2-7.6 (60 °C, 59 g/L in H2O, after autoclaving)

solubility

59 g/L

bulk density

700 kg/m3

application(s)

clinical
food and beverages
microbiology

storage temp.

15-25°C

suitability

Yersinia spp.

General description

for the selective cultivation of Yersinia

Analysis Note

Appearance (clearness)clearAppearance (colour)redpH-value (25 °C)7.2 - 7.6Typical composition (g/litre): Peptone from casein10.0; Peptone from meat 10.0; Yeast extract 2.0; D(-)Mannitol 20.0 ; Sodium pyruvate 2.0; Sodium chloride 1.0; Magnesium sulfate 0.01; Bile salt mixture1.0; Neutral red 0.03; Crystal violet 0.001; Agar-agar 12.5.Growth promotion test in accordance with ISO 11133.
Base tested with supplement Art. 1.16466.Inoculum (Yersinia enterocolitica ATCC 9610 (WDCM 00038))10⁴Inoculum (Yersinia enterocolitica ATCC 23715 (WDCM 00160))10⁴Inoculum (Escherichia coli ATCC 25922 (WDCM 00013))10⁴Inoculum (Escherichia coli ATCC 8739 (WDCM 00012))10⁴Inoculum (Salmonella typhimurium ATCC 14028 (WDCM 00031))10⁴Inoculum (Staphylococcus aureus ATCC 25923 (WDCM 00034))10⁴Growth (Yersinia enterocolitica ATCC 23715 (WDCM 00160))good to very goodGrowth (Yersinia enterocolitica ATCC 9610 (WDCM 00038))good to very goodGrowth (Escherichia coli ATCC 25922 (WDCM 00013))none to poorGrowth (Escherichia coli ATCC 8739 (WDCM 00012))none to poorGrowth (Salmonella typhimurium ATCC 14028 (WDCM 00031))noneGrowth (Staphylococcus aureus ATCC 25923 (WDCM 00034))nonered centre (Yersinia enterocolitica ATCC 9610 (WDCM 00038))+red centre (Yersinia enterocolitica ATCC 23715 (WDCM 00160))+Incubation: 24 hrs.; 30 °C; aerobic.
Reference: Tryptic soy agar. Strains tested with three-loop-streak.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Warning

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

11 - Combustible Solids

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WGK 3


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