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1.32647

Sigma-Aldrich

Alcian blue staining kit, pH 1.0

for the detection of strongly sulfated mucins for microscopy

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Quality Level

IVD

for in vitro diagnostic use

pH

1.0

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

General description

The ready-to-use Alcian blue stainig kit, pH 1.0 - for the detection of strongly sulfated mucins - for microscopy, can be used for this staining in histological tissue. Alcian blue pH 1.0 is used to stain strongly sulfated mucins in human-histological specimen materials, for example histological sections of e. g. colon or bronchus. The dye Alcian blue 8 GX (Product number 1.01647) is a water-soluble phthalocyanine dye. Acidic mucins have a negative charge and bind the positively charged Alcian blue 8 GX staining dye.
A solution of hydrochloric acid and Alcian blue is used for the staining procedure. At a strongly acidic pH of 1.0, this solution suppresses the dissociation of the carboxyl groups and selectively stains only the strongly sulfated mucins and polyanions. Counterstaining takes place with a nuclear fast red/aluminium sulfate solution. The undifferentiated method (Alcian blue 8 GX solution, pH 2.5) enables only the detection of acidic glycoproteins, e. g. mucins, without distinguishing between carboxyl and sulfate groups.
The package is sufficient for approx. 2500 applications. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.

Application

For the detection of strongly sulfated mucins.

Analysis Note

Nuclei: red
Strongly sulfated mucins: light blue

pictograms

Corrosion

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 2


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