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14-749-M

Sigma-Aldrich

TAO1 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. For use in Kinase Assays.

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eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

assay

93% (Coomassie blue staining, SDS-PAGE)

specific activity

1179 U/mg

mol wt

Mw 41 kDa

manufacturer/tradename

Chemicon®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

Gene Information

human ... TAOK1(57551)

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HIPK1 Protein, active, 10 µg

assay

93% (Coomassie blue staining, SDS-PAGE)

assay

84% (Coomassie blue staining, SDS-PAGE)

assay

84% (Coomassie blue staining, SDS-PAGE)

assay

-

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 41 kDa

mol wt

Mw 50.2 kDa

mol wt

Mw 138.3 kDa

mol wt

Mw 49.6 kDa

specific activity

1179 U/mg

specific activity

915 U/mg

specific activity

1562 U/mg

specific activity

-

General description

N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327.
Product Source: Expressed by baculovirus in Sf21 cells.

Application

Research Category
Metabolism
Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Biochem/physiol Actions

Protein Target: TAO1
Target Sub-Family: STE

Quality

Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO1.

Unit Definition

Specific Activity: where one unit of TAO1 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.

Physical form

10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose

Storage and Stability

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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M Hutchison et al.
The Journal of biological chemistry, 273(44), 28625-28632 (1998-10-24)
Several components of the budding yeast pheromone-response pathway are conserved in mammalian mitogen-activated protein (MAP) kinase pathways. Thus, we used degenerate oligonucleotides derived from the sequence of the Saccharomyces cerevisiae protein kinase Ste20p to amplify related sequences from the rat.

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