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1.51466

Supelco

Chromolith® RP-18 endcapped

L × I.D. 100 mm x 4.6 mm HPLC columns Validation Kit (3 columns from 3 different batches)

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Synonym(s):
Chromolith® Performance RP-18 endcapped 100-4.6 HPLC columns Validation Kit (3 columns from 3 different batches)

material

PEEK column

Quality Level

agency

suitable for USP L1

product line

Chromolith®

feature

endcapped

packaging

pkg of 3 ea

extent of labeling

18% Carbon loading

parameter

200 bar pressure
45 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 4.6 mm

matrix

monolithic silica

matrix active group

C18 bonding phase

pore size

13 nm mesoporosity
2 μm macroporosity

operating pH range

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

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1.520631.520621.52006
Chromolith® RP-18 endcapped L × I.D. 100 mm x 4.6 mm HPLC columns Validation Kit (3 columns from 3 different batches)

1.51466

Chromolith® RP-18 endcapped

Chromolith® RP-18 endcapped L × I.D. 100 mm x 3 mm HPLC columns Validation Kit (3 columns from 3 different batches)

1.52063

Chromolith® RP-18 endcapped

Chromolith® RP-18 endcapped L × I.D. 50 mm x 2 mm HPLC columns Validation Kit (3 columns from 3 different batches)

1.52062

Chromolith® RP-18 endcapped

Chromolith® RP-18 endcapped L × I.D. 100 mm x 2 mm HPLC column

1.52006

Chromolith® RP-18 endcapped

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

matrix active group

C18 bonding phase

technique(s)

HPLC: suitable, mass spectrometry (MS): suitable (High), LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

1.5 μm macroporosity, 13 nm mesoporosity

pore size

1.5 μm macroporosity, 13 nm mesoporosity

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

General description

Chromolith® Performance RP-18 endcapped 100-4.6 HPLC columns Validation Kit consists of 3 columns from 3 different manufacturing batches. Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure. Chromolith® Performance RP-18 endcapped columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), and the surface modification is octadecylsilane with full end-capping. Chromolith® Performance RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® Performance RP-18 endcapped columns are perfectly suitable for LC-MS applications.The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.The revolutionary, monolithic silica with its unique combination of macropores and mesopores enable:
  • Rapid separations at very low column back-pressure
  • High matrix tolerance, allowing time and cost savings with simplified workflows
  • Cost-savings due to extended column lifetimes
  • Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are: Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032] Or Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451] with the Chromolith® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]. The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467] Need more efficiency? Chromolith® High Resolution RP-18 endcapped 100-4.6 HPLC columns [1.52022] provide a column efficiency exceeding 140,000 plates/meter. Learn more:Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC ColumnsAll Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Extent of testing: passes test

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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