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Supelco

Chromolith® WP 300 Protein A

L × I.D. 25 mm x 4.6 mm HPLC column

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Synonym(s):
Chromolith® WP 300 Protein A 25-4.6 HPLC column

material

PEEK column

product line

Chromolith®

feature

endcapped: no
endcapped: no

packaging

1 ea of

parameter

2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure

technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable

column L × I.D.

2.5 cm × 4.6 mm

surface area

120 m2/g

matrix

monolithic silica
monolithic silica

matrix active group

Protein A bonding phase
Protein A bonding phase

pore size

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

operating pH range

1.5-7.5

operating pH

1.5-7.5

separation technique

affinity

storage temp.

2-8°C
2-8°C

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1 of 4

This Item
1.523581.522651.52271
Chromolith® WP 300 Protein A L × I.D. 25 mm x 4.6 mm HPLC column

1.52258

Chromolith® WP 300 Protein A

Chromolith® WP 300 Protein A L × I.D. 25 mm × 2 mm HPLC column

1.52358

Chromolith® WP 300 Protein A

Chromolith® WP 300 RP-8 L × I.D. 100 mm x 4.6 mm HPLC column

1.52265

Chromolith® WP 300 RP-8

Chromolith® WP 300 RP-18 L × I.D. 50 mm x 4.6 mm HPLC column

1.52271

Chromolith® WP 300 RP-18

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

separation technique

affinity

separation technique

-

separation technique

reversed phase

separation technique

reversed phase

product line

Chromolith®

product line

-

product line

Chromolith®

product line

Chromolith®

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 mm mesoporosity, 30 nm mesoporosity

General description

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Related Content

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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