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217595

Sigma-Aldrich

Anti-CD40L Mouse mAb (24-31)

liquid, ≥95% (SDS-PAGE), clone 24-31

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Synonym(s):
Anti-gp39

biological source

mouse

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

24-31, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

0.5 mg/mL gentamycin sulfate as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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Sigma-Aldrich

217595

Anti-CD40L Mouse mAb (24-31)

Sigma-Aldrich

217590

Anti-CD40 Mouse mAb (EA-5)

Sigma-Aldrich

217640

Anti-CD11a Mouse mAb (38)

Sigma-Aldrich

217669

Anti-CD28 Mouse mAb (ANC28.1/5D10)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

24-31, monoclonal

clone

EA-5, monoclonal

clone

38, monoclonal

clone

ANC28.1/5D10, monoclonal

form

liquid

form

liquid

form

liquid

form

liquid

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

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General description

Anti-CD40L, mouse monoclonal, clone 24-32, recognizes the ~36 kDa CD40L (gp39) on activated CD3+ human PBL. It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.
Note: 1 T = 1 test.
Protein A affinity purified mouse monoclonal antibody. Recognizes the ~39-40 kDa CD40L protein.
Recognizes the ~36 kDa CD40L (gp39) protein on activated CD3+ human PBL.

Immunogen

Human
human sgp39 fusion protein

Application

Flow Cytometry (10 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

Immunoblotting (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Other Notes

This antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x105 ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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