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217640

Sigma-Aldrich

Anti-CD11a Mouse mAb (38)

liquid, clone 38, Calbiochem®

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Synonym(s):
Anti-α L Integrin, Anti-LFA-1, Anti-αL Integrin, Anti-LFA-1

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

38, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Anti-CD11a, mouse monoclonal, clone 38, recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 cells. It is validated for FC, IP, IHC on frozen sections and for cell inhibition assays.
Protein A purified mouse monoclonal antibody. Recognizes the I domain of the ~180 kDa integrin α L subunit (CD11a).
Recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 human tumor cells.

Application

Cell Inhibition Assays (1-5 µg/ml, see comments)

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Molt-4 cells

Other Notes

Also blocks binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured Molt-4 human tumor cells using anti-CD11a antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. Variables associated with assay conditions will dictate the proper working dilution.
Holness, C.L., et al. 1995. J. Biol. Chem. 270, 877.
Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.
Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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