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Anti-CD28 Mouse mAb (ANC28.1/5D10)

liquid, >95% (immunoglobulin, SDS-PAGE), clone ANC28.1/5D10

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biological source


Quality Level

antibody form

purified antibody

antibody product type

primary antibodies


ANC28.1/5D10, monoclonal


>95% (immunoglobulin, SDS-PAGE)




500 μg/mL gentamycin sulfate as preservative

species reactivity




storage condition

OK to freeze



shipped in

wet ice

storage temp.


target post-translational modification


Gene Information

human ... CD28(940)

General description

Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.
Protein A purified mouse monoclonal antibody. Recognizes the CD28 protein.
Recognizes CD28 on human T cells and BHK cells transfected with human CD28.


human CD28 Ig fusion protein and HPB-MLT human tumor cells


Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see comments)


Please refer to vial label for lot-specific concentration.


Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.


Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Other Notes

Kariv, I., et al. 1996. J. Immunol.157, 29.
61st Forum in Immunology. 1995. Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol.5, 361.
Monomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids




Not applicable


Not applicable

Certificates of Analysis (COA)

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Renée M Marshall et al.
Journal of immunology (Baltimore, Md. : 1950), 175(10), 6402-6411 (2005-11-08)
Stimulation of primary human T lymphocytes results in up-regulation of cyclin T1 expression, which correlates with phosphorylation of the C-terminal domain of RNA polymerase II (RNAP II). Up-regulation of cyclin T1 and concomitant stabilization of cyclin-dependent kinase 9 (CDK9) may
Mengting Chen et al.
International journal of biological sciences, 19(16), 5160-5173 (2023-11-06)
Rosacea is a common inflammatory skin disorder mediated by the dysregulation of both keratinocytes and T cells. Here, we report that aquaporin 3 (AQP3), a channel protein that mediates the transport of water/glycerol, was highly expressed in the epidermis and
Zijun Wang et al.
The Journal of clinical investigation, 130(7), 3717-3733 (2020-03-20)
T follicular helper (Tfh) cells are indispensable for the formation of germinal center (GC) reactions, whereas T follicular regulatory (Tfr) cells inhibit Tfh-mediated GC responses. Aberrant activation of Tfh cells contributes substantially to the pathogenesis of autoimmune diseases, such as
Xiaofei Gao et al.
Frontiers in immunology, 13, 799331-799331 (2022-03-18)
Trace element iron affects T cell biology, but the knowledge about the role of iron in regulating Treg cell expansion is limited. Treg cells play an important role in keeping peripheral T cell tolerance, increasing Treg cell expansion is a
Ruifang Wu et al.
The Journal of clinical investigation, 128(6), 2551-2568 (2018-05-15)
Immune imbalance of T lymphocyte subsets is a hallmark of psoriasis, but the molecular mechanisms underlying this aspect of psoriasis pathology are poorly understood. Here, we report that microRNA-210 (miR-210), a miR that is highly expressed in both psoriasis patients

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