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218765

Sigma-Aldrich

Caspase-9 Substrate I, Fluorogenic

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6).

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Synonym(s):
Caspase-9 Substrate I, Fluorogenic, Ac-LEHD-AFC
Empirical Formula (Hill Notation):
C33H38F3N7O11
CAS Number:
Molecular Weight:
765.69

Quality Level

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

white

solubility

DMSO: 5 mg/mL

fluorescence

λex ~400 nm
λem ~505 nm

shipped in

ambient

storage temp.

−20°C

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688225218739218763
Sigma-Aldrich

218765

Caspase-9 Substrate I, Fluorogenic

Sigma-Aldrich

218739

Caspase-1 Substrate X, Fluorogenic

form

solid

form

solid

form

lyophilized

form

lyophilized

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze, protect from light, desiccated

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, desiccated, protect from light

General description

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Biochem/physiol Actions

Cell permeable: no
Primary Target
A fluorogenic substrate for caspase-9
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Leu-Glu-His-Asp-AFC

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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