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23-025-M

Sigma-Aldrich

UbcH4 Protein, active, 50 µg

Active, full length, recombinant human UbcH4. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

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Synonym(s):
Ubiquitin-conjugating enzyme E2 D2, Ubiquitin-protein ligase D2, UbcH5b, Ubiquitin-conjugating enzyme E2-17kDa 2, UBE2D2
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

recombinant

expressed in E. coli

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

NCBI accession no.

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This Item
23-035-M23-029-M23-036-M
recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

NCBI accession no.

NM_003339

NCBI accession no.

NM_181888

NCBI accession no.

NM_003338

NCBI accession no.

NM_003341

Quality Level

100

Quality Level

100, 300

Quality Level

100, 300

Quality Level

100, 300

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

General description

C-terminal cMyc and 6His-tagged, recombinant human Smac/DIABLO amino acids 56-end, expressed in E.coli
Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate. The UbcH5 family (UbcH5a, UbcH5b/UbcH4 and UbcH5c) have been shown to be the most active class of E2 enzymes in cell extracts and are associated with the regulation of a number of proteins associated with cell signalling, including p53, IκBα and β-catenin.

Application

Active, full length, recombinant human UbcH4. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Ubiquitination Cascade Comoponent: E2

Physical form

Purified using immobilized metal affinity chromatography.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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