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341635

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Fibronectin, Human Plasma

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Synonym(s):
Fibronectin, Human Plasma, Fibronectin purified from human plasma, Human Fibronectin
CAS Number:

form

liquid

Quality Level

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−20°C

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341635

Fibronectin, Human Plasma

vibrant-m

F1141

Fibronectin bovine plasma

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Fibronectin, Rat Plasma

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Fibronectin, Bovine Plasma

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

form

liquid

form

solution

form

liquid

form

liquid

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

storage condition

-

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

General description

Fibronectin is a glycoprotein belonging to the extracellular matrix (ECM) protein family. Fibronectin contains two polypeptides (α and β chains) linked by two disulfide bonds near the carboxyl termini to form a dimer.Native fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.

Application

The human plasma fibronectin has been used for coating surfaces such as poly-l-lysine (PLL)-coated nanochips, polyacrylamide (PAAm) hydrogel substrates, glass slides and more, providing a substrate for the adhesion and culture of cells in various experimental setups.

Biochem/physiol Actions

Human fibronectin protein plays crucial roles in various cellular behaviors such as cell attachment, migration, embryogenesis, wound healing, tissue integrity, and malignant processes. One of the key features enabling fibronectin to support cell adhesion is the presence of the Arg-Gly-Asp (RGD) tripeptide. It has been extensively studied in areas like fibronectin coating on polystyrene (PS) and poly(lactic-co-glycolic acid) (PLGA) surfaces. Additionally, fibronectin has been the subject of significant research regarding its involvement in regulating processes such as differentiation-to-growth switching, tubulin monomer growth, cell cycle status, cell spreading, and motility.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.3.

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Thaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Contaminants: none detected by IEP with antiserum to fibronectin or normal human serum
single major band by SDS-PAGE

Other Notes

Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol.59, 1.
Krejci, K., and Fritz, H. 1976. FEBS Lett.64, 152.


Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem.283, 2297.


Brown, S., et al. 2002. Nature418, 200.


Kim, M., FEBS Letters581 1917.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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