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368057

Sigma-Aldrich

Granzyme B Substrate I, Colorimetric

Colorimetric substrate for the detection of granzyme B and caspase-8 activity.

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Synonym(s):
Granzyme B Substrate I, Colorimetric, Ac-IETD- pNA, Caspase-8 Substrate I, Ac-IETD-pNA, Caspase-8 Substrate I
Empirical Formula (Hill Notation):
C27H38N6O12
Molecular Weight:
638.62

Quality Level

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

white

solubility

DMSO: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

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This Item
218805565764264151
assay

≥95% (HPLC)

assay

≥98% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

DMSO: 5 mg/mL

solubility

DMSO: 5 mg/mL

solubility

DMSO: 5 mg/mL, 5% acetic acid: soluble

solubility

DMSO: 5 mg/mL

General description

Colorimetric substrate for detection of granzyme B. Cleavage is monitored colorimetrically at ~405 nm.
Colorimetric substrate for the detection of granzyme B and caspase-8 activity. Cleavage of pNA is monitored colorimetrically at ~405 nm.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Colorimetric substrate for the detection of granzyme B and caspase-8 activity
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Ile-Glu-Thr-Asp-pNA

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Koeplinger, K.A., et al. 2000. Protein Exp. Purif. 18, 378.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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