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α-Secretase Substrate II, Fluorogenic

An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP).

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Synonym(s):
α-Secretase Substrate II, Fluorogenic, Ac-RE(EDANS)-VHHQKLVF-K(DABCYL)-R-OH
Empirical Formula (Hill Notation):
C100H144N30O20S
Molecular Weight:
2118.47

Quality Level

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

red

solubility

DMSO: 5 mg/mL

fluorescence

λex ~340 nm
λem ~490 nm

shipped in

ambient

storage temp.

−20°C

General description

An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP). Shown to be a substrate for yapsin 2, a glycosyl-phosphatidylinositol-linked yeast aspartyl protease (Vmax/Km = 0.031 min-1). The cleavage is shown to occur at the Lys-Leu bond.
An internally quenched fluorogenic peptide substrate that contains the α-secretase cleavage site of β-amyloid precursor protein (APP). Shown to be an effective substrate for yapsin-2, a glycosyl-phosphatidylinositol-linked aspartyl protease found in yeast (Vmax/Km = 0.031 min-1). The cleavage has been shown to occur at the Lys-Leu bond. Excitation max: ~340 nm; emission max: ~490 nm.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP)
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Arg-Glu(EDANS)-Val-His-His-Gln-Lys-Leu-Val-Phe-Lys(DABCYL)-Arg-OH

Other Notes

Komano, H., et al. 1999. J. Biol. Chem. 274, 24431.
Komano, H., et al. 1998. J. Biol. Chem. 273, 31648.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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