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69755-M

Millipore

His•Bind® Buffer Kit

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manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

storage temp.

2-8°C

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This Item
GE11-0034-0070666539720
vibrant-m

69755-M

His•Bind® Buffer Kit

vibrant-m

GE11-0034-00

His Buffer Kit

vibrant-m

70666

Ni-NTA His•Bind® Resin

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

-

storage condition

do not freeze

storage condition

OK to freeze

General description

The His•Bind Buffer Kit is a set of pre-tested buffers designed for use with IDA-based His•Bind Resin for convenient, rapid one-step purification of proteins by metal chelation chromatography. Solutions are included for Ni2+ charging, binding, washing and elution of up to ten 2.5 ml columns.

Components

•2 × 80 ml8X Binding Buffer

•25 ml8X Wash Buffer

•50 ml4X Elute Buffer

•50 ml4X Strip Buffer

•20 ml8X Charge Buffer

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation

target_organs

respiratory tract irritation

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3


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Articles

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

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