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AB5156

Sigma-Aldrich

Anti-Calcium Channel Antibody, Voltage Gated α 1C

Chemicon®, from rabbit

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Synonym(s):
L-type
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, rabbit, mouse, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CACNA1C(775)

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This Item
MABN863MAB13170AB5230
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

Gene Information

human ... CACNA1C(775)

Gene Information

human ... CACNA1C(775)

Gene Information

human ... CACNA1C(775)
rabbit ... Cacna1C(100101555)

Gene Information

human ... CACNB3(784)

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

species reactivity

rat, rabbit, mouse, human

species reactivity

human

species reactivity

mouse, human

species reactivity

rat

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General description

Voltage-sensitive calcium channels (vscc) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1c gives rise to l-type calcium currents. Long-lasting (l-type) calcium channels belong to the "high-voltage activated" (hva) group. They are blocked by dihydropyridines (dhp), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin- gvia (omega-ctx-gvia) and omega-agatoxin-IVA (omega-aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to dhp compounds (Swiss Prot).

Specificity

Recognizes alpha 1C subunit (both LMW and HMW forms) of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. Guinea pig (17/18 residues identical); human and rabbit (16/18 residues identical).

Immunogen

Purified peptide (C)TTKI NMDDL QPSEN EDKS (CNC1) from 848-865 of alpha 1C subunit of rat brain voltage-gated calcium channel (Accession number P22002). Epitope location is in the intracellular loop between domains II and III.

Application

Alpha 1 subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

Immunohistochemistry: 1:200 on rat brain sections.

Western blot: 1:200 using ECL on rat brain and heart material. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.

Immunoprecipitation

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions and protocols must be determined by the end user.
Detect Calcium Channel using this Anti-Calcium Channel Antibody, Voltage Gated α 1C, validated for use in IH, IP & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Signaling Neuroscience

Target description

190 & 210 kDa

Physical form

Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
ImmunoAffinity Purified

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution add glycerol (ASC grade or better) at a ratio of 1:1 and maintain at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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State-dependent signaling by Cav1.2 regulates hair follicle stem cell function.
Yucel, G; Altindag, B; Gomez-Ospina, N; Rana, A; Panagiotakos, G; Lara, MF; Dolmetsch, R; Oro, AE
Genes & Development null
David Jerome et al.
The European journal of neuroscience, 36(8), 3134-3141 (2012-07-06)
Early odor preference learning in rats provides a simple model for studying learning and memory. Learning results in an enhanced output from mitral cells, which carry odor information from the olfactory bulb to the olfactory cortex. Mitral cell NMDA receptors
Huan Chen et al.
Nature communications, 11(1), 4328-4328 (2020-08-30)
The general anesthetic ketamine has been repurposed by physicians as an anti-depressant and by the public as a recreational drug. However, ketamine use can cause extensive pathological changes, including ketamine cystitis. The mechanisms of ketamine's anti-depressant and adverse effects remain
Wei Wang et al.
Molecular medicine reports, 14(4), 3848-3854 (2016-09-08)
Remodeling of atrial electrophysiology and structure is the primary feature of atrial fibrillation (AF). Evidence suggests that abnormalities in the expression levels of embryological cardiovascular development‑associated transcription factors, including Nkx2.5, are crucial for the development of AF. Rat atrial myocardial
R A Morton et al.
Neuroscience, 238, 59-70 (2013-02-19)
Voltage-gated calcium channels (VGCCs) play a major role during the development of the central nervous system (CNS). Ca(2+) influx via VGCCs regulates axonal growth and neuronal migration as well as synaptic plasticity. Specifically, L-type VGCCs have been well characterized to

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