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AB5312

Sigma-Aldrich

Anti-Growth Associated Protein 43 Antibody

serum, Chemicon®

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Synonym(s):
GAP-43, Neuromodulin, B-50
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, monkey, rat, feline, bovine

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GAP43(2596)

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This Item
AB5220AB5401MAB347
antibody form

serum

antibody form

purified antibody

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

species reactivity

mouse, human, monkey, rat, feline, bovine

species reactivity

mouse (high degree), rat, bovine, chicken, human

species reactivity

rat

species reactivity

feline, rat, hamster

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General description

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Specificity

AB5312 specifically recognizes GAP-43 regardless of the protein′s phosphorylation state. This antiserum has been refered to in the literature as CG-1.

Immunogen

Purified GAP-43 from feline brain (McIntosh and Parkinson, 1990).

Application

Immunocytochemistry: 1:100-1:400 for mouse primary neurons, NTera2 neurons.

Immuohistochemistry: 1:2,000 on coronal sections of adult visual cortex (McIntosh and Parkinson, 1990). AB5312 has been used to stain GAP-43 in 4% paraformaldehyde-fixed paraffin-embedded tissue sections of mouse olfactory bulb (Legrier, 2001).

Western blot: 1:500 - 1:1,000. Detects a single band at approximately 50 kDa on western blots of protein extracts from adult visual cortex (McIntosh and Parkinson, 1990).

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuroregenerative Medicine

Signaling Neuroscience
This Anti-Growth Associated Protein 43 Antibody is validated for use in IC, IH, IH(P), IP, WB for the detection of Growth Associated Protein 43.

Target description

23.6 kDa

Physical form

Serum. Liquid with 0.02% sodium azide added as a preservative.
Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Roland Blumer et al.
Investigative ophthalmology & visual science, 58(12), 5105-5121 (2017-10-08)
To analyze in a frontal-eyed mammal (cat) the postnatal development of palisade endings in extraocular muscles (EOMs) and to compare the spatiotemporal and quantitative patterns of palisade endings among individual rectus muscles. Cats of different ages ranging from birth to
Yuetong Ding et al.
PloS one, 9(7), e101918-e101918 (2014-07-09)
Brachial plexus injury (BPI) and experimental spinal root avulsion result in loss of motor function in the affected segments. After root avulsion, significant motoneuron function is restored by re-implantation of the avulsed root. How much this functional recovery depends on
Jing Zhou et al.
Journal of cellular and molecular medicine, 18(2), 326-343 (2014-01-01)
Stem cell transplantation represents a promising strategy for the repair of spinal cord injury (SCI). However, the low survival rate of the grafted cells is a major obstacle hindering clinical success because of ongoing secondary injury processes, which includes excitotoxicity
Xiaolong Zheng et al.
iScience, 26(11), 108306-108306 (2023-11-29)
Human pluripotent stem cell (hPSC)-derived neurons have shown promise in treating spinal cord injury (SCI). We previously showed that hPSC-derived dorsal spinal γ-aminobutyric acid (GABA) neurons can alleviate spasticity and promote locomotion in rats with SCI, but their long-term safety

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